各种荧光染料的吸收波长和激发波长
2012-7-11 5:16:32 点击：
| 荧光分子 |
颜色
|
激发波长
|
发射波长
|
| Alexa Fluor 350 |
Blue
|
346 nm
|
442 nm
|
| Alexa Fluor 405 |
Blue
|
402 nm
|
421 nm
|
| Pacific Blue |
Blue
|
410 nm
|
455 nm
|
| DAPI |
Blue
|
340 nm
|
488 nm
|
| AMCA |
Blue
|
350 nm
|
450 nm
|
| Cascade Blue |
Blue
|
400 nm
|
420 nm
|
| EviTag(TM) quantum dots - Lake Placid Blue (490 nm) |
Blue
|
<400 nm
|
490 nm
|
| Fluoroblue |
Blue
|
350 nm
|
450 nm
|
| Image-Blue |
Blue
|
380 nm
|
465 nm
|
| Marina Blue |
Blue
|
365 nm
|
460 nm
|
| Pacific Blue |
Blue
|
410 nm
|
455 nm
|
| Cy2 |
Green
|
491 nm
|
509 nm
|
| Alexa Fluor 430 |
Green
|
434 nm
|
539 nm
|
| Alexa Fluor 488 |
Green
|
495 nm
|
519 nm
|
| Alexa Fluor 488 FluoroNanogold |
Green
|
495 nm
|
519 nm
|
| Alexa Fluor 500 |
Green
|
503 nm
|
525 nm
|
| Alexa Fluor 514 |
Green
|
518 nm
|
540 nm
|
| FITC |
Green
|
490 nm
|
520 nm
|
| Fluorescein |
Green
|
494 nm
|
518 nm
|
| HNP |
Green
|
290 nm
|
509 nm
|
| 5-FAM |
Green
|
494 nm
|
522 nm
|
| 6-FAM |
Green
|
492 nm
|
517 nm
|
| BODIPY FL |
Green
|
505 nm
|
513 nm
|
| CMNB-caged Fluorescein |
Green
|
494 nm
|
518 nm
|
| Cyanine 2 |
Green
|
492 nm
|
510 nm
|
| DTAF |
Green
|
495 nm
|
520 nm
|
| DTAF/FITC |
Green
|
495 nm
|
520 nm
|
| dUTP-Biotin and Avidin FITC |
Green
|
495 nm
|
520 nm
|
| dUTP-FITC |
Green
|
495 nm
|
520 nm
|
| EviTag(TM) quantum dots - Adirondack Green (520 nm) |
Green
|
<400 nm
|
520 nm
|
| EviTag(TM) quantum dots - Catskill Green (540 nm) |
Green
|
<400 nm
|
540 nm
|
| FITC (Fluorescein) |
Green
|
495 nm
|
520 nm
|
| Fluorescein-5-EX, succinimidyl ester (FSE) |
Green
|
495 nm
|
520 nm
|
| Hi FITC |
Green
|
495 nm
|
520 nm
|
| JOE |
Green
|
520 nm
|
548 nm
|
| MFP488 |
Green
|
501 nm
|
523 nm
|
| Oregon Green 488 |
Green
|
496 nm
|
524 nm
|
| Oregon Green 514 |
Green
|
511 nm
|
530 nm
|
| Qdot 525 |
Green
|
488 nm
|
515 nm
|
| TET |
Green
|
521 nm
|
536 nm
|
| CAL Fluor(R) Gold 540 |
Green
|
522 nm
|
544 nm
|
| NL493 |
Green
|
493 nm
|
514 nm
|
| GFP |
Green
|
395/475 nm
|
509 nm
|
| Cy3 |
Yellow
|
548 nm
|
562 nm
|
| Cy3B |
Yellow
|
558 nm
|
572 nm
|
| Alexa Fluor 532 |
Yellow
|
531 nm
|
554 nm
|
| Alexa Fluor 546 |
Yellow
|
556 nm
|
573 nm
|
| Alexa Fluor 555 |
Yellow
|
555 nm
|
565 nm
|
| R-Phycoerythrin |
Yellow
|
480, 546, 565 nm
|
578 nm
|
| B-Phycoerythrin |
Yellow
|
546, 565 nm
|
575 nm
|
| HNP/Fast Red TR |
Yellow
|
290 nm
|
562 nm
|
| 5-TAMRA |
Yellow
|
546 nm
|
574 nm
|
| Atto 550 |
Yellow
|
554 nm
|
576 nm
|
| CAL Fluor(R) Orange 560 |
Yellow
|
538 nm
|
559 nm
|
| Cyanine 3 |
Yellow
|
550 nm
|
570 nm
|
| DyLight 547 |
Yellow
|
547 nm
|
574 nm
|
| EviTag(TM) quantum dots - Birch Yellow (580 nm) |
Yellow
|
<400 nm
|
580 nm
|
| EviTag(TM) quantum dots - Hops Yellow (560 nm) |
Yellow
|
<400 nm
|
560 nm
|
| HEX |
Yellow
|
535 nm
|
556 nm
|
| NED |
Yellow
|
546 nm
|
575 nm
|
| Qdot 565 |
Yellow
|
<500 nm
|
565 nm
|
| Quasar(R) 570 |
Yellow
|
548 nm
|
566 nm
|
| R-Phycoerythrin (R-PE) |
Yellow
|
488 nm
|
575 nm
|
| TRITC (Rhodamine) |
Yellow
|
550 nm
|
570 nm
|
| VIC |
Yellow
|
538 nm
|
554 nm
|
| NL557 |
Yellow
|
557 nm
|
574 nm
|
| Phycoerythrin |
Yellow
|
480-566 nm
|
575 nm
|
| Cy3.5 |
Orange
|
581 nm
|
596 nm
|
| Alexa Fluor 568 |
Orange
|
578 nm
|
603 nm
|
| ROX |
Orange
|
575 nm
|
602 nm
|
| DyeMer 488/605 |
Orange
|
502 nm
|
609 nm
|
| DyeMer 488/615 |
Orange
|
502 nm
|
615 nm
|
| CAL Fluor(R) Red 590 |
Orange
|
569 nm
|
591 nm
|
| CAL Fluor(R) Red 610 |
Orange
|
569 nm
|
610 nm
|
| Cyanine 3.5 |
Orange
|
581 nm
|
596 nm
|
| DyeMer 488/605 |
Orange
|
488 nm
|
605 nm
|
| DyeMer 488/615 |
Orange
|
488 nm
|
615 nm
|
| EviTag(TM) quantum dots - Fort Orange (600 nm) |
Orange
|
<400 nm
|
600 nm
|
| Lissamine Rhodamine |
Orange
|
570 nm
|
590 nm
|
| MFP555 |
Orange
|
560 nm
|
585 nm
|
| Qdot 585 |
Orange
|
532 nm
|
585 nm
|
| Qdot 605 |
Orange
|
<600 nm
|
605 nm
|
| ROX |
Orange
|
575 nm
|
602 nm
|
| R-Phycoerythrin-Texas Red (PE-Texas Red, ECD) |
Orange
|
480-565 nm
|
615 nm
|
| Rhodamine Red-X |
Orange
|
570 nm
|
590 nm
|
| TMR red |
Red
|
540 (520-560) nm
|
580 (570-620) nm
|
| Cy5 |
Red
|
646 nm
|
664 nm
|
| Cy5.5 |
Red
|
673 nm
|
692 nm
|
| CypHer5E |
Red
|
644 nm
|
663 nm
|
| Alexa Fluor 594 |
Red
|
590 nm
|
617 nm
|
| Alexa Fluor 594 FluoroNanogold |
Red
|
590 nm
|
617 nm
|
| Alexa Fluor 610 |
Red
|
612 nm
|
628 nm
|
| Alexa Fluor 610–R-PE |
Red
|
496, 546, 565 nm
|
630 nm
|
| Alexa Fluor 633 |
Red
|
632 nm
|
647 nm
|
| Alexa Fluor 635 |
Red
|
633 nm
|
647 nm
|
| Alexa Fluor 647 |
Red
|
650 nm
|
668 nm
|
| Alexa Fluor 647–R-phycoerythrin |
Red
|
496, 546, 565 nm
|
668 nm
|
| Alexa Fluor 660 |
Red
|
663 nm
|
690 nm
|
| Texas Red |
Red
|
596 nm
|
620 nm
|
| DyeMer 488/630 |
Red
|
502 nm
|
630 nm
|
| Allophycocyanin |
Red
|
650 nm
|
660 nm
|
| PI |
Red
|
535 nm
|
617 nm
|
| Allophycocyanin (APC) |
Red
|
650 nm
|
660 nm
|
| Allophycocyanin-Cyanine 5 |
Red
|
633 nm
|
670 nm
|
| Allophycocyanin-Cyanine 5.5 |
Red
|
633 nm
|
690 nm
|
| Atto 647 |
Red
|
645 nm
|
669 nm
|
| CAL Fluor(R) Red 635 |
Red
|
618 nm
|
637 nm
|
| Cyanine 5 |
Red
|
650 nm
|
670 nm
|
| DyLight 647 |
Red
|
647 nm
|
673 nm
|
| DyeMer 488/630 |
Red
|
488 nm
|
630 nm
|
| Dyomics 647 |
Red
|
660 nm
|
673 nm
|
| Lightcycler Red- 640 |
Red
|
625 nm
|
640 nm
|
| PBXL-1 |
Red
|
545 nm
|
666 nm
|
| PBXL-3 |
Red
|
614 nm
|
662 nm
|
| PerCP (Peridinin Chlorophyll) |
Red
|
490 nm
|
675 nm
|
| PerCP-Cy5.5 |
Red
|
488 nm
|
695 nm
|
| Qdot 655 |
Red
|
<620 nm
|
655 nm
|
| Quantum Red |
Red
|
488 nm
|
660 nm
|
| Quasar(R) 670 |
Red
|
647 nm
|
667 nm
|
| R-Phycocyanin (R-PC) |
Red
|
614 nm
|
647 nm
|
| R-Phycoerythrin-Cyanine 5 (Cy-Chrome, PC5, Tri-Color, Spectral Red) |
Red
|
488 nm
|
660 nm
|
| Sulforhodamine |
Red
|
585 nm
|
620 nm
|
| Texas Red |
Red
|
596 nm
|
620 nm
|
| Cyanine Q (Cyanine 5.18) |
Red
|
488 nm
|
670 nm
|
| NL637 |
Red
|
637 nm
|
658 nm
|
| Allophycocyanin |
Red
|
620-650 nm
|
660-670 nm
|
| CytoCy5S |
Red
|
628 nm
|
638 nm
|
| Alexa Fluor 680 |
Far-Red
|
679 nm
|
702 nm
|
| Alexa Fluor 680–Allophycocyanin |
Far-Red
|
650 nm
|
702 nm
|
| Alexa Fluor 680-R-phycoerythrin |
Far-Red
|
555 nm
|
700 nm
|
| Alexa Fluor 700 |
Far-Red
|
702 nm
|
723 nm
|
| Alexa Fluor 700–Allophycocyanin |
Far-Red
|
650 nm
|
723 nm
|
| Alexa Fluor 750 |
Far-Red
|
749 nm
|
775 nm
|
| Alexa Fluor 750–Allophycocyanin |
Far-Red
|
650 nm
|
775 nm
|
| Allophycocyanin-Cyanine 7 |
Far-Red
|
633 nm
|
760 nm
|
| Cyanine 5.5 |
Far-Red
|
678 nm
|
703 nm
|
| Cyanine 7 |
Far-Red
|
710 nm
|
805 nm
|
| IRDYE700DX |
Far-Red
|
689 nm
|
700 nm
|
| IRDYE800 |
Far-Red
|
778 nm
|
795 nm
|
| IRDYE800CW |
Far-Red
|
774 nm
|
800 nm
|
| NIRD700 |
Far-Red
|
682 nm
|
701 nm
|
| Qdot 705 |
Far-Red
|
<700 nm
|
705 nm
|
| Qdot 800 |
Far-Red
|
<600 nm
|
800 nm
|
| R-Phycoerythrin-Cyanine 5.5 |
Far-Red
|
488 nm
|
703 nm
|
| R-Phycoerythrin-Cyanine 7 (PC7) |
Far-Red
|
488 nm
|
805 nm |
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